1.
Electron. j. biotechnol
;
12(2): 12-13, Apr. 2009. ilus, tab
Article
in English
| LILACS
| ID: lil-551373
ABSTRACT
The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.